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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans
Chapitres
- 00:04Introduction
- 00:30Microfluidic Flow System Assembly
- 02:05Microfluidic Flow System for in vivo FPOP
- 04:13In vivo FPOP
- 05:52Results: IV-FPOP Oxidative Modification of Proteins within C. elegans
- 07:37Conclusion
In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.
