Journal
/
Génétique
/
Universell og effektiv elektroporasjonsprotokoll for genteknologi av gastrointestinale organoider
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Universell og effektiv elektroporasjonsprotokoll for genteknologi av gastrointestinale organoider

15,884 Views

•

07:19 min

•

February 18, 2020

DOI:

10.3791/60704-v

DOI:
10.3791/60704-v

•

07:19 min
February 18, 2020

•

11 Views
, , , , , ,
1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

Tags

ElectroporationGenetic EngineeringOrganoids3D Cell CultureTransfectionUniversal ProtocolEfficientTumor OrganoidsHealthy OrganoidsSpheroids2D Cell CultureDissociationY-27632Basal MediumElectroporation BufferPlasmid DNAImpedanceElectroporation Parameters

Read Article

Cite this Article

Gaebler, A., Hennig, A., Buczolich, K., Weitz, J., Welsch, T., Stange, D. E., Pape, K. Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids. J. Vis. Exp. (156), e60704, doi:10.3791/60704 (2020).

Download .ris file

Copy

Share Video

.

Copy