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消化管オルガノイドの遺伝子工学のための汎用的かつ効率的なエレクトロポレーションプロトコル
Journal JoVE
Génétique
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Journal JoVE Génétique
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
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消化管オルガノイドの遺伝子工学のための汎用的かつ効率的なエレクトロポレーションプロトコル

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07:19 min

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February 18, 2020

DOI:

10.3791/60704-v

DOI:
10.3791/60704-v

•

07:19 min
February 18, 2020

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11 Views
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1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

Tags

ElectroporationGenetic EngineeringOrganoids3D Cell CultureTransfectionUniversal ProtocolEfficientTumor OrganoidsHealthy OrganoidsSpheroids2D Cell CultureDissociationY-27632Basal MediumElectroporation BufferPlasmid DNAImpedanceElectroporation Parameters

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Gaebler, A., Hennig, A., Buczolich, K., Weitz, J., Welsch, T., Stange, D. E., Pape, K. Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids. J. Vis. Exp. (156), e60704, doi:10.3791/60704 (2020).

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