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Protocole d’électroporation universel et efficace pour le génie génétique des organoïdes gastro-intestinaux

Journal JoVE
Génétique

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Journal JoVE Génétique

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

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Protocole d’électroporation universel et efficace pour le génie génétique des organoïdes gastro-intestinaux

15,884 Views

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07:19 min

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February 18, 2020

DOI:

10.3791/60704-v

DOI:

10.3791/60704-v

•

07:19 min

February 18, 2020

•

11 Views

Anne-Marlen Gaebler, Alexander Hennig, Katharina Buczolich, Jürgen Weitz, Thilo Welsch, Daniel E. Stange, Kristin Pape

¹Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

Tags

Electroporation Genetic Engineering Organoids 3D Cell Culture Transfection Universal Protocol Efficient Tumor Organoids Healthy Organoids Spheroids 2D Cell Culture Dissociation Y-27632 Basal Medium Electroporation Buffer Plasmid DNA Impedance Electroporation Parameters

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