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Purkinje Cell Survival i Organotypic lillehjernen Slice kulturer
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Neurosciences
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Journal JoVE Neurosciences
Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Purkinje Cell Survival i Organotypic lillehjernen Slice kulturer

DOI:
10.3791/60353-v

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06:31 min

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December 18, 2019

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1Department of Physiology,Northwestern University, Feinberg School of Medicine

Chapitres

  • 00:04Titre
  • 00:58Organotypic Cerebellar Slice Culture Preparation
  • 01:40Organotypic Cerebellar Slice Culture Dissection
  • 03:32Immunofluorescence
  • 05:24Results: Representative Purkinje Cell Survival in Organotypic Cerebellar Slice Culture Following High Potassium Chloride (KCl) Treatment
  • 05:54Conclusion

Summary

Traduction automatique

Traduction automatique

Organotypic skive kulturer er et kraftig verktøy for å studere neurodevelopmental eller degenerative/regenererende prosesser. Her beskriver vi en protokoll som modeller den neurodevelopmental død Purkinje celler i mus lillehjernen skive kulturer. Denne metoden kan ha nytte forskning i nervecellene narkotika funn.

Tags

Keywords: Purkinje CellOrganotypic Slice CultureCerebellar SlicesNeuroprotectionNeurodegenerative DiseasesCell-cell ConnectionsIn Vivo ConditionsPrimary Cell CultureCerebellumParasagittal Sections

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