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大肠杆菌-表达自组装蛋白纳米颗粒的生产，用于需要三聚体异体的疫苗
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
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大肠杆菌-表达自组装蛋白纳米颗粒的生产，用于需要三聚体异体的疫苗

DOI:
10.3791/60103-v

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00:10 min

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August 21, 2019

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, , ,
1U.S. Military HIV Research Program,Walter Reed Army Institute of Research, 2Henry M. Jackson Foundation for the Advancement of Military Medicine, 3Alpha-O Peptides AG

Chapitres

  • 00:04Titre
  • 00:43Lysis of E. Coli BL21(DE3) Containing Six-Helix Bundle Self-assembling Protein Nanoparticles
  • 01:24Protein Purification
  • 05:14Purity Assessment and Protein Identification
  • 06:28SHB-SAPN Refolding
  • 07:46Dynamic Light Scattering (DLS)
  • 08:30Results: Representative SHB-SAPN Characterization
  • 10:07Conclusion

Summary

Traduction automatique

Traduction automatique

这里提供了一种详细的方法，描述了用于疫苗开发中的自组装蛋白纳米颗粒（SDNA）的纯化、再折叠和表征。

Tags

E. ColiSelf-assembling Protein NanoparticlesSAPNVaccinesTrimeric Epitope PresentationProtein PurificationProtein RefoldingProtein CharacterizationSix-helix BundleFPLCAffinity ChromatographyHisTrap HP ColumnProtein ExpressionLarge-scale ProductionClinical Trials

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