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A Method to Study de novo Formation of Chromatin Domains
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Journal JoVE Génétique
A Method to Study de novo Formation of Chromatin Domains

A Method to Study de novo Formation of Chromatin Domains

5,302 Views

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00:07 min

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August 23, 2019

DOI:

10.3791/60039-v

DOI:
10.3791/60039-v

•

00:07 min
August 23, 2019

•

12 Views
,
1Howard Hughes Medical Institute,New York University School of Medicine, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Whitehead Institute for Biomedical Research

Summary

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This method is designed to follow formation of PRC2-mediated chromatin domains in cell lines, and the method can be adapted to many other systems.

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Chromatin DomainsPRC2CRISPREEDMouse Embryonic Stem CellsGenotypingDonor Template DNA

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Cite this Article

Oksuz, O., Reinberg, D. A Method to Study de novo Formation of Chromatin Domains. J. Vis. Exp. (150), e60039, doi:10.3791/60039 (2019).

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