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Onderzoek van de kern dynamiek van de mitotische en Meiotische splijtstof gist door fluorescentie-Live-cel microscopie
Journal JoVE
Biologie
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Journal JoVE Biologie
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Onderzoek van de kern dynamiek van de mitotische en Meiotische splijtstof gist door fluorescentie-Live-cel microscopie

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12:04 min

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June 24, 2019

DOI:

10.3791/59822-v

DOI:
10.3791/59822-v

•

12:04 min
June 24, 2019

•

7 Views
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1Program in Molecular and Computational Biology,University of Southern California, 2Leonard Davis School of Gerontology,University of Southern California

Summary

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Hier presenteren we live-celbeeldvorming die een niet-toxische microscopie methode is die onderzoekers in staat stelt om eiwit gedrag en nucleaire dynamiek in levende splijting gistcellen te bestuderen tijdens mitose en meiosis.

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Live Cell MicroscopyFission YeastNuclear DynamicsMitosisMeiosisFluorescence ImagingCell PreparationAgarose PadMicroscope Slide SetupTime-lapse Imaging

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Escorcia, W., Shen, K., Yuan, J., Forsburg, S. L. Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy. J. Vis. Exp. (148), e59822, doi:10.3791/59822 (2019).

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