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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
JoVE 杂志
生物学
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JoVE 杂志 生物学
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

9,060 Views

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12:04 min

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June 24, 2019

DOI:

10.3791/59822-v

DOI:
10.3791/59822-v

•

12:04 min
June 24, 2019

•

7 Views
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1Program in Molecular and Computational Biology,University of Southern California, 2Leonard Davis School of Gerontology,University of Southern California

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Here, we present live-cell imaging which is a non-toxic microscopy method that allows researchers to study protein behavior and nuclear dynamics in living fission yeast cells during mitosis and meiosis.

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Live Cell MicroscopyFission YeastNuclear DynamicsMitosisMeiosisFluorescence ImagingCell PreparationAgarose PadMicroscope Slide SetupTime-lapse Imaging

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Escorcia, W., Shen, K., Yuan, J., Forsburg, S. L. Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy. J. Vis. Exp. (148), e59822, doi:10.3791/59822 (2019).

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