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Brug af Near-Infrared fluorescens og høj opløsning scanning til at måle protein Expression i gnaver hjernen
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Neurosciences
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Journal JoVE Neurosciences
Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Brug af Near-Infrared fluorescens og høj opløsning scanning til at måle protein Expression i gnaver hjernen

DOI:
10.3791/59685-v

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06:04 min

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May 23, 2019

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, , ,
1Department of Psychological and Brain Sciences,University of Delaware, 2Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Chapitres

  • 00:04Titre
  • 00:45Single Immunohistochemical Reaction
  • 02:45Imaging and Protein Expression Analysis
  • 04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
  • 05:30Conclusion

Summary

Traduction automatique

Traduction automatique

Her præsenterer vi en protokol, der bruger nær-infrarøde farvestoffer sammen med Immunhistokemi og høj opløsning scanning til assay proteiner i hjernen regioner.

Tags

Near-infrared FluorescenceHigh-resolution ScanningProtein ExpressionRodent BrainImmunohistochemistryMolecular Signaling PathwaysAMPA/NMDA RatioCryostatParaformaldehydeTBSPrimary AntibodySecondary AntibodyNear-infrared Scanning

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