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蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)

50,665 Views

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10:00 min

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June 20, 2019

DOI:

10.3791/59615-v

DOI:
10.3791/59615-v

•

10:00 min
June 20, 2019

•

92 Views
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1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Summary

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该协议描述了尺寸排除色谱与多角度光散射(SEC-MALS)的结合,用于对溶液中的蛋白质和复合物进行绝对表征。SEC-MALS 确定纯蛋白质、原生寡聚物、异质复合物和修饰性蛋白质(如糖蛋白)的分子量和大小。

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Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).

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