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Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
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06:58 min
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May 27, 2019
DOI:
1Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude,Universidade do Porto, 2Instituto de Biologia Celular e Molecular,Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar, 4Departamento de Biologia, Faculdade de Ciências,Universidade do Porto
Summary
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Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.
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Flores, C., Tamagnini, P. Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins. J. Vis. Exp. (147), e59590, doi:10.3791/59590 (2019).
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