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Biochemistry

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JoVE Journal Biochemistry
Looking Outwards
 

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Article DOI: 10.3791/59590-v • 06:58 min • May 27th, 2019
May 27th, 2019 •
1,2,3, 1,2,4
1Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude, Universidade do Porto, 2Instituto de Biologia Celular e Molecular, Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar, 4Departamento de Biologia, Faculdade de Ciências, Universidade do Porto

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Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.

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Cyanobacterial Carbohydrate Polymers Proteins Secretion Mechanisms Extracellular Customize Biotechnological Applications Biomedical Applications Protocols Straightforward Tailored Producing Organism User Needs Application Bacteria Easy Bacterial Strain Purity Required Cultivate Growth Measurement Dialysis Membranes Deionized Water Stirring Centrifuge Supernatant Pellet Contaminants
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