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激发-扫描高光谱成像显微镜，以有效区分荧光信号

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Ingénierie

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Journal JoVE Ingénierie

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

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激发-扫描高光谱成像显微镜，以有效区分荧光信号

7,839 Views

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00:07 min

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August 22, 2019

DOI:

10.3791/59448-v

DOI:

10.3791/59448-v

•

00:07 min

August 22, 2019

•

13 Views

Joshua Deal^1,2,3, Andrea Britain^2,3, Thomas Rich^2,3, Silas Leavesley^1,2,3

¹Department of Chemical and Biomolecular Engineering,University of South Alabama, ²Center for Lung Biology,University of South Alabama, ³Department of Pharmacology,University of South Alabama

Summary

光谱成像已成为在单个样品中识别和分离多个荧光信号的可靠解决方案，并可轻松区分感兴趣的信号和背景或自荧光。激励扫描高光谱成像通过减少必要的图像采集时间，同时提高信噪比，改进了该技术。

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