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Imaging 3D ad alta risoluzione dell'infezione da virus della rabbia nel tessuto cerebrale con la rimozione del solvente

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

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Imaging 3D ad alta risoluzione dell'infezione da virus della rabbia nel tessuto cerebrale con la rimozione del solvente

10,620 Views

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09:42 min

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April 30, 2019

DOI:

10.3791/59402-v

DOI:

10.3791/59402-v

•

09:42 min

April 30, 2019

•

3 Views

Luca Zaeck, Madlin Potratz, Conrad M. Freuling, Thomas Müller, Stefan Finke

¹Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut,Federal Research Institute for Animal Health

Tags

3D Imaging Rabies Virus Immunostaining Solvent Clearing Virus Infection Cellular Context Vibratome Sectioning Methanol Pretreatment Bleaching Immunostaining Permeabilization Blocking Primary Antibody Labeling

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