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Biologie du développement
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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

DOI:
10.3791/59359-v

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06:21 min

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April 25, 2019

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, ,
1Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

Chapitres

  • 00:04Titre
  • 00:58Human Induced Pluripotent Stem Cell (iPSC) Pre-induction Preparation
  • 02:14Presomitic Mesoderm (PSM) and Somitic (SM) Differentiation
  • 03:20SM Derivative [Syndetome (SYN)] Differentiation
  • 04:28Results: Representative iPSC-derived Product Characterization
  • 05:44Conclusion

Summary

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We present here a protocol for the differentiation of human induced pluripotent stem cells into each somite derivative (myotome, sclerotome, dermatome, and syndetome) in chemically defined conditions, which has applications in future disease modeling and cell-based therapies in orthopedic surgery.

Tags

Induced Pluripotent Stem CellsIPSCSomite DerivativesMyotomeDermatomeSclerotomeSyndetomeParaxial MesodermPresomitic MesodermDelta-like Protein 1Regenerative TherapyDisease ModelingMusculoskeletal DisorderHuman Embryogenesis

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