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在异种性鼠模型中的移植-无源疾病的诱导和评分,以及使用数字PCR对小鼠组织中人类T细胞的定量
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Immunologie et infection
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Journal JoVE Immunologie et infection
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
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在异种性鼠模型中的移植-无源疾病的诱导和评分,以及使用数字PCR对小鼠组织中人类T细胞的定量

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06:06 min

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May 23, 2019

DOI:

10.3791/59107-v

DOI:
10.3791/59107-v

•

06:06 min
May 23, 2019

•

2 Views
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1Department of Microbiology, Molecular Genetics, and Immunology,University of Kansas Medical Center

Summary

Automatically generated

在这里,我们提出了一个协议,以诱导和评分疾病在异种移植-宿主疾病(xenoGVHD)模型。xenoGVHD为研究人类T细胞的免疫抑制提供了一个体内模型。此外,我们介绍如何使用数字PCR检测组织中的人类T细胞,作为量化免疫抑制的工具。

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Seng, A., Markiewicz, M. A. Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR. J. Vis. Exp. (147), e59107, doi:10.3791/59107 (2019).

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