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Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

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06:24 min

February 13, 2019

DOI:

06:24 min
February 13, 2019

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Summary

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Fluorescence resonance energy transfer-based real-time observation systems of the DNA strand exchange reaction mediated by Rad51 were developed. Using the protocols presented here, we are able to detect the formation of reaction intermediates and their conversion into products, while also analyzing the enzymatic kinetics of the reaction.

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