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一种从人类死后小脑中的激光捕获微解剖 purkinje 细胞中分离高质量 rna 的不锈钢协议
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09:04 min
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January 17, 2019
DOI:
1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University
Summary
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该协议采用无污渍的方法, 通过激光捕获微解剖, 将新鲜冷冻组织中的 purkinje 细胞从人类死后的小脑中观察和分离。该协议的目的是为 rna 测序生成足够数量的高质量 rna。
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Cite this Article
Martuscello, R. T., Louis, E. D., Faust, P. L. A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum. J. Vis. Exp. (143), e58953, doi:10.3791/58953 (2019).
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