Here, we present a protocol for in vivo validation of hydrogel-based cell therapy, illustrated by the example of islet transplantation. h-Omental Matrix Islet filliNG (hOMING) implantation allows implantation of a cell-hydrogel mixture between the omental layers, near to blood vessels, to maximize engraftment in a proper metabolic environment.
Schaschkow, A., Mura, C., Pinget, M., Bouzakri, K., Maillard, E. Intra-Omental Islet Transplantation Using h-Omental Matrix Islet filliNG (hOMING). J. Vis. Exp. (145), e58898, doi:10.3791/58898 (2019).