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Neuroscience

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JoVE Journal Neuroscience
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine
 

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Article DOI: 10.3791/58873-v • 10:48 min • January 25th, 2019
January 25th, 2019 •
1, 2, 3, 3, 1
1Department of Pharmacology, Northwestern University Feinberg School of Medicine, 2Department of Physiology, Northwestern University Feinberg School of Medicine, 3Janelia Research Campus, Howard Hughes Medical Institute

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This article presents a method for studying nicotinic acetylcholine receptors (nAChRs) in mouse brain slices by nicotine uncaging. When coupled with simultaneous patch clamp recording and 2-photon laser scanning microscopy, nicotine uncaging connects nicotinic receptor function with cellular morphology, providing a deeper understanding of cholinergic neurobiology.

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Probing Nicotinic Acetylcholine Receptor Mouse Brain Slices Laser Flash Photolysis Photoactivatable Nicotine Multi-photon Laser-scanning Microscopy Cholinergic Receptors Receptor Functional Expression Patterns Cholinergic Synaptic Transmission Volume Transmission Programmable Pipette Puller Glass Micropipette Recording Solution Photoactivatable Nicotine Local Application Micropipette Pipette Holder Micromanipulator Pressure Ejection System Extracellular Recording Solution
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