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Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
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Génétique
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Journal JoVE Génétique
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

DOI:
10.3791/58682-v

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07:50 min

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November 21, 2018

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, , ,
1Biochemistry Department,Boston University School of Medicine, 2Bioinformatic Hub,Boston University

Chapitres

  • 00:04Titre
  • 00:34Dissection and Preparation of BAT for Chromatin Immunoprecipitation
  • 03:29Immune Complexes Collection and DNA Recovery
  • 05:51Results: ChIP Validation by qPCR
  • 07:17Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.

Tags

Keywords: Chromatin ImmunoprecipitationChIPMouseFormaldehyde Cross-linkingCell LysisSonicationImmunoprecipitationAntibodyProtein A Agarose

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