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生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
DOI:

10:44 min

August 09, 2019

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Chapitres

  • 00:04Titre
  • 00:57Preparation of OP9/DLL1 Cells for Co-culture with iPSC
  • 02:08In Vitro Differentiation of iPSC into Immature T Cells
  • 05:003D Thymic Organ Culture to Generate iTE
  • 08:00Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
  • 09:46Conclusion

Summary

Traduction automatique

本文介绍了一种通过三维(3D)胸腺培养系统生成肿瘤抗原特异性诱导多能干细胞衍生胸腺(iTE)的新方法。iTE 是 T 细胞的同质子集,与天真的 T 细胞密切相关,具有增殖、记忆形成和肿瘤抑制的能力。

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