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利用磁性纳米粒子丰富和扩大罕见抗原特异性 t 细胞

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Ingénierie

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Journal JoVE Ingénierie

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

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利用磁性纳米粒子丰富和扩大罕见抗原特异性 t 细胞

11,268 Views

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09:28 min

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November 17, 2018

DOI:

10.3791/58640-v

DOI:

10.3791/58640-v

•

09:28 min

November 17, 2018

•

6 Views

John W. Hickey^1,2,3,4, Jonathan P. Schneck^2,4

¹Department of Biomedical Engineering, School of Medicine,Johns Hopkins University, ²Institute for Cell Engineering, School of Medicine,Johns Hopkins University, ³Institute for Nanobiotechnology,Johns Hopkins University, ⁴Department of Pathology, School of Medicine,Johns Hopkins University

Summary

抗原特异性 t 细胞由于其频率极低, 很难在治疗中表征或利用。在此, 我们提供了一个协议, 以开发一个磁性粒子, 它可以结合到抗原特异性 t 细胞, 以丰富这些细胞, 然后扩大他们的几百倍, 用于表征和治疗。

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