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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

DOI:
10.3791/58631-v

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10:10 min

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January 17, 2019

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, , ,
1Department of Chemistry and Biotechnology, School of Engineering,University of Tokyo, 2Riken Center for Emergent Matter Science

Chapitres

  • 00:04Titre
  • 00:29Preparation of Guests with CagedGlue-R Tags
  • 02:17Preparation of Hep3B Cell Sample for Microscopic Observations
  • 02:59Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
  • 04:53Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
  • 05:48Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
  • 07:04Cell Viability Assay
  • 08:20Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
  • 09:51Conclusion

Summary

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This protocol describes light-triggered nuclear translocation of guests in living cells using caged molecular glue tags. This method is promising for site-selective nuclear-targeting drug delivery.

Tags

Nuclear TranslocationCaged Molecular GluePhotoactivatable TagsQuantum DotDrug DeliveryHEP3B CellsFluorescent DyeEndosomes

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