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利用 tap 标签纯化叶绿体转移蛋白配合物的亲和纯化
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
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利用 tap 标签纯化叶绿体转移蛋白配合物的亲和纯化

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07:01 min

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November 01, 2018

DOI:

10.3791/58532-v

DOI:
10.3791/58532-v

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07:01 min
November 01, 2018

•

2 Views
, , ,
1Laboratory of Plant Physiology,University of Neuchatel, 2Institut fur Biochemie und Biotechnologie,Martin-Luther-Universitat Halle-Wittenberg

Summary

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我们在这里提出了一个经过验证和测试的方案, 以纯化叶绿体蛋白进口复合物 (toc-tic 复合物) 使用 tap-tag 标签。一步亲和力隔离协议有可能应用于任何蛋白质, 并可通过质谱技术识别新的相互作用伙伴。

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Cite this Article

Shanmugabalaji, V., Douet, V., Agne, B., Kessler, F. Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag. J. Vis. Exp. (141), e58532, doi:10.3791/58532 (2018).

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