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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

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07:01 min

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November 01, 2018

DOI:

10.3791/58532-v

DOI:
10.3791/58532-v

•

07:01 min
November 01, 2018

•

2 Views
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1Laboratory of Plant Physiology,University of Neuchatel, 2Institut fur Biochemie und Biotechnologie,Martin-Luther-Universitat Halle-Wittenberg

Summary

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We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

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ChloroplastTransloconProtein ComplexesTAP TagTOCTICArabidopsisAffinity PurificationMembrane FractionsDetergentSolubilizationUltracentrifugationGrinding BufferCentrifugationHomogenization

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Shanmugabalaji, V., Douet, V., Agne, B., Kessler, F. Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag. J. Vis. Exp. (141), e58532, doi:10.3791/58532 (2018).

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