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Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography
Journal JoVE
Biologie
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Journal JoVE Biologie
Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography
DOI:

06:21 min

October 09, 2018

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Chapitres

  • 00:04Titre
  • 00:52Sample Preparation Protocol
  • 02:19Micro-CT Scanning Protocol
  • 03:03“Image Analysis for a Single CT Imageof a Maize Stem or Leaf”
  • 04:06Image Analysis Protocol for CT Image Series of a Maize Root
  • 05:09Results: Phenotypic Traits of Vascular Bundles in Maize Stems, Leaves, and Roots Computed
  • 05:35Conclusion

Summary

Traduction automatique

We provide a novel method to improve the X-ray absorption contrast of maize tissue suitable for ordinary microcomputed tomography scanning. Based on CT images, we introduce a set of image-processing workflows for different maize materials to effectively extract microscopic phenotypes of vascular bundles of maize.

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