Journal
/
Neurosciences
/
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

13,461 Views

•

08:30 min

•

January 18, 2019

DOI:

10.3791/58479-v

DOI:
10.3791/58479-v

•

08:30 min
January 18, 2019

•

10 Views
, , , , , ,
1Department of Pain,First Affiliated Hospital of Nanchang University, 2Department of Pediatrics,First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Explore More Videos

Acute Spinal Cord SlicesWhole-cell Patch-clamp RecordingSubstantia Gelatinosa NeuronsSensory TransmissionNociceptive RegulationChronic PainAgar BlockSucrose-based ACSFTranscardial PerfusionSpinal Cord ExtractionLumbosacral EnlargementMeninges RemovalPia Arachnoid MembraneVentral/dorsal RootsTransverse SlicesParasagittal SlicesVibratome

Read Article

Cite this Article

Zhu, M., Zhang, D., Peng, S., Liu, N., Wu, J., Kuang, H., Liu, T. Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons. J. Vis. Exp. (143), e58479, doi:10.3791/58479 (2019).

Download .ris file

Copy

Share Video

.

Copy