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Separazione di spinaci Thylakoid complessi proteici da nativo verde Gel elettroforesi e caratterizzazione di banda utilizzando Time-Correlated Single Photon Counting
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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
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Separazione di spinaci Thylakoid complessi proteici da nativo verde Gel elettroforesi e caratterizzazione di banda utilizzando Time-Correlated Single Photon Counting

8,064 Views

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08:40 min

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February 14, 2019

DOI:

10.3791/58470-v

DOI:
10.3791/58470-v

•

08:40 min
February 14, 2019

•

3 Views
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1Department of Plant Biology,Michigan State University, 2Department of Chemistry,Michigan State University

Summary

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Qui presentiamo un protocollo per separare solubilizzate thylakoid complessi mediante elettroforesi in Gel verde nativo. Gel verde bande successivamente sono caratterizzati da tempo correlato Single Photon Counting (TCSPC) e vengono forniti i passaggi base per l'analisi dei dati.

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Cite this Article

Schwarz, E., Blanchard, G. Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting. J. Vis. Exp. (144), e58470, doi:10.3791/58470 (2019).

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