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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

DOI:
10.3791/58470-v

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08:40 min

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February 14, 2019

•

,
1Department of Plant Biology,Michigan State University, 2Department of Chemistry,Michigan State University

Chapitres

  • 00:04Titre
  • 00:50Isolation of Crude Thylakoid Membranes from Spinach Leaves
  • 02:51Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
  • 04:02Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
  • 05:01Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
  • 06:07Results: Native Green Gel Electrophoresis and Band Characterization
  • 08:14Conclusion

Summary

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Traduction automatique

Here we present a protocol to separate solubilized thylakoid complexes by Native Green Gel electrophoresis. Green gel bands are subsequently characterized by Time Correlated Single Photon Counting (TCSPC) and basic steps for data analysis are provided.

Tags

Thylakoid Protein ComplexesNative Green Gel ElectrophoresisTime-correlated Single Photon CountingPhotosynthesis ResearchChlorophyllSpinach LeavesTMK BufferMethanol ExtractionCentrifugationSample Preparation

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