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利用本地绿色凝胶电泳分离菠菜类黄体蛋白配合物, 用时间相关单光子计数表征
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Journal JoVE Environnement
Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
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利用本地绿色凝胶电泳分离菠菜类黄体蛋白配合物, 用时间相关单光子计数表征

8,063 Views

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08:40 min

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February 14, 2019

DOI:

10.3791/58470-v

DOI:
10.3791/58470-v

•

08:40 min
February 14, 2019

•

3 Views
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1Department of Plant Biology,Michigan State University, 2Department of Chemistry,Michigan State University

Summary

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在这里, 我们提出了一个协议, 以分离溶解性类囊化复合物的本地绿色凝胶电泳。绿色凝胶带随后通过时间相关单光子计数 (tcspc) 进行了表征, 并提供了数据分析的基本步骤。

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Schwarz, E., Blanchard, G. Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting. J. Vis. Exp. (144), e58470, doi:10.3791/58470 (2019).

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