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Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
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Biologie du développement
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Journal JoVE Biologie du développement
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

DOI:
10.3791/58370-v

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12:48 min

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January 07, 2019

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, , , ,
1Department of Biomedicine,University Hospital and University of Basel, 2Division of Cardiology,University Hospital Basel

Chapitres

  • 00:04Titre
  • 01:01Tissue Preparation and Digestion
  • 02:48Filtration
  • 04:43Primary Culture of Isolated SP-CPCs
  • 05:28SP-CPC Growth and Viability Under Different Serum Concentrations
  • 07:28Induction of Endothelial Differentiation
  • 08:59Tube Formation Assay
  • 10:15Résultats
  • 11:58Conclusion

Summary

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This protocol describes an endothelial differentiation technique for cardiac progenitor cells. It particularly focuses on how serum concentration and cell-seeding density affect the endothelial differentiation potential.

Tags

Cardiac Progenitor CellsEndothelial DifferentiationLow Serum ConditionsCell TherapyCardiac DiseaseAnesthetizeCollagenase BHank’s Balanced Salt SolutionRed Blood Cell Lysis Buffer

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