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“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
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“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
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10:25 min
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February 19, 2019
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Summary
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The goal of this protocol is to provide a step-by-step guide to perform 3-D "liver-on-a-chip" infection experiments with the hepatitis B virus.
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Liver-on-a-chipPrimary HepatocytesKupffer CellsHepatitis B VirusViral PathogenesisInfection KineticsViral ReplicationImmune InvasionPhysiological ModelDrug TestingHepatic MicroenvironmentHepatotropic InfectionsLiver DiseasesDrug MetabolismLong-term CultureSpecialized ToolsVisual DemonstrationMembraneMicrofluidic PlatesSeedingMedium Priming
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Ortega-Prieto, A. M., Skelton, J. K., Cherry, C., Briones-Orta, M. A., Hateley, C. A., Dorner, M. "Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection. J. Vis. Exp. (144), e58333, doi:10.3791/58333 (2019).
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