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A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
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Biochimie
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Journal JoVE Biochimie
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

DOI:
10.3791/58038-v

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05:47 min

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July 29, 2018

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, ,
1Department of Chemical Engineering,University of Florida

Chapitres

  • 00:04Titre
  • 00:47Preparing Cells for Imaging
  • 01:29Microscopy and Image Acquisition
  • 04:02Results: Characterization of the Nuclear Deformation
  • 05:29Conclusion

Summary

Traduction automatique

Traduction automatique

In this protocol, we describe a micropipette method to directly apply a controlled force to the nucleus in a living cell. This assay allows interrogation of nuclear mechanical properties in the living, adherent cell.

Tags

Nuclear MechanicsNuclear DeformationNucleo-cytoskeleton IntegrationNuclear Mechanical PropertiesAdherent CellsProbing ForceMicromanipulatorMicropipetteHutchinson-Gilford Progerial CellsNIH-3T3 Fibroblasts

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