Journal
/
Environnement
/
A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying
Journal JoVE
Environnement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Environnement
A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

DOI:
10.3791/57892-v

•

04:52 min

•

June 01, 2018

•

, ,
1College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, 2UNEP-Tongji Institute of Environment for Sustainable Development,Tongji University

Chapitres

  • 00:05Titre
  • 00:52Designing of an Arraying Template and Zebrafish Embryo Spawning
  • 02:18Arraying Zebrafish Embryos into a 96-well Plate
  • 03:48Results: Representative Microscopic Images of Embryos After 6-well Plate Transferrence
  • 04:24Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to design and fabricate a zebrafish embryo arraying template, followed by a detailed procedure on the use of such template for high throughput zebrafish embryo arraying into a 96-well plate.

Tags

3D-printed TemplateHigh ThroughputZebrafish EmbryoArrayingAutomationZebrafish Screening96-well Plate384-well PlateEmbryo EntrapmentVacuum-based ArrayingTemplate Design3D PrintingZebrafish SpawningEmbryo CollectionEmbryo Selection

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce (Lactuca sativa) Germplasm Collections

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Basic Research in Plasma Medicine – A Throughput Approach from Liquids to Cells

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.)

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Evaluation of the Productivity of Social Wasp Colonies (Vespinae) and an Introduction to the Traditional Japanese Vespula Wasp Hunting Technique

Read Article