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Neuroscience

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JoVE Journal Neuroscience
Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
 

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Article DOI: 10.3791/57879-v • 09:46 min • September 21st, 2021
September 21st, 2021 •
*1, *2, 3, 4, 1, 2, 2
1Research Unit of Experimental Neurotraumatology, Department of Neurosurgery, Medical University Graz, 2Department of Neurology, Medical University Graz, 3Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, 4Division of Vascular Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet
* These authors contributed equally

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The protocol presented here allows the reproduction of a widespread grey matter demyelination of both cortical hemispheres in adult male Dark Agouti rats. The method comprises of intracerebral implantation of a catheter, subclinical immunization against myelin oligodendrocyte glycoprotein, and intracerebral injection of a pro-inflammatory cytokine mixture through the implanted catheter.

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Keywords: Rat Cerebral Cortex Demyelination Pro-inflammatory Cytokines Multiple Sclerosis Catheter Implantation Stereotactic Surgery Cortical Inflammation Gray Matter Demyelination Anchor Screws
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