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粗病毒提取中 DNA 的组合分析及对感染叶 RNA 的分析发现新的病毒基因组
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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粗病毒提取中 DNA 的组合分析及对感染叶 RNA 的分析发现新的病毒基因组

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08:56 min

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July 27, 2018

DOI:

10.3791/57855-v

DOI:
10.3791/57855-v

•

08:56 min
July 27, 2018

•

1 Views
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1Texas A&M Agrilife Center at Dallas, 2Noble Research Center,Oklahoma State University, 3Department of Biology, College of Engineering and Natural Sciences,The University of Tulsa, 4Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology,Oklahoma State University

Summary

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在这里, 我们提出了一种新的方法来识别植物病毒的双链 DNA 基因组。我们使用标准方法从受感染的树叶中提取 DNA 和 RNA, 并进行下一代测序。生物信息学工具将序列组合成重叠, 确定重叠代表病毒基因组并将基因组分配给分类组。

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DNA AnalysisCrude Virion ExtractionRNA AnalysisInfected LeavesNew Virus GenomesEnvironmental VirologyBadnavirusesHomogenizationDifferential CentrifugationBio-analyzerLaboratory ProtocolLeaf GrindingUreaDetergentCentrifugationPellet SeparationVirus PurificationDNA Sequencing

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Verchot, J., Thapa, A., Wijayasekara, D., Hoyt, P. R. Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes. J. Vis. Exp. (137), e57855, doi:10.3791/57855 (2018).

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