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Chapitres
- 00:04Titre
- 00:54Preparation of Precursor Solutions (and Optional Encapsulant Solutions)
- 01:50Silica Synthesis
- 02:48Acid Elution of the Materials and Silica Separation and Drying
- 03:53Silicomolybdic Acid Assay on Monomeric Silica Species
- 04:37Bradford Assay Procedure for Determination of Protein Concentration in Silica
- 05:28Results: Analysis of Functional Silica Prepared via a Bioinspired Method
- 06:56Conclusion
Here, we present a protocol to synthesize bioinspired silica materials and immobilize enzymes therein. Silica is synthesized by combining sodium silicate and an amine 'additive', which neutralize at a controlled rate. Material properties and function can be altered either by in situ enzyme immobilization or post-synthetic acid elution of encapsulated additives.
