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Biologie du développement
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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
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Biologie du développement
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Journal JoVE Biologie du développement
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

DOI:
10.3791/57599-v

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09:33 min

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June 02, 2018

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, , ,
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine

Chapitres

  • 00:05Titre
  • 00:42Preparation of Mouse Embryonic Day 10.5 Pancreatic Progenitors
  • 06:12Culturing Dissociated Progenitors to Form Pancreatoids
  • 06:50Results: Generation of Pancreatoids that Retain Native Mesenchyme and Develop Endocrine Cells
  • 08:37Conclusion

Summary

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Traduction automatique

Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.

Tags

PancreatoidsPancreatic ProgenitorsScaffold-freeThree-dimensionalInsulin-expressingMouseEndocrine CellsMesenchymeDissectionE10.5DispaseTrypsinIACUCUterusYolk SacPBS

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