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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

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11:56 min

May 04, 2018

DOI:

11:56 min
May 04, 2018

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Summary

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The protocol presents the Escherichia coli-based selective pressure incorporation of non-canonical amino acids (ncAAs) into the lactococcal antimicrobial peptide nisin. Its properties can be changed during recombinant expression via substitution with desired ncAAs in defined growth media. Resulting changes in bioactivity are mapped by growth inhibition assays and fluorescence microscopy.

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