/
/
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
9,651 Views
•
11:13 min
•
May 25, 2018
DOI:
Summary
Automatically generated
Here, we describe methods to perform ChIP-Seq and CARIP-Seq, including library preparation for next-generation sequencing, to generate global epigenomic and chromatin-associated RNA maps in ES cells.
Explore More Videos
CARIP-SeqChIP-SeqChromatin-associated RNAsProtein-DNA InteractionsEmbryonic Stem CellsEpigeneticsTranscription FactorsEpigenetic RegulatorsHistone ModificationsChromatin ImmunoprecipitationSonicationFormaldehyde Cross-linkingGlycinePhosphate Buffered SalineTrypsinizationMEF MediumFetal Bovine SerumSonication BufferSodium Dodecyl Sulfate
Read Article
Cite this Article
Kidder, B. L. CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells. J. Vis. Exp. (135), e57481, doi:10.3791/57481 (2018).
Copy