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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
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June 23, 2018
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Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.
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Carija, A., Ventura, S., Navarro, S. Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast. J. Vis. Exp. (136), e57470, doi:10.3791/57470 (2018).
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