Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
9,193 Views
•
12:29 min
•
March 20, 2018
DOI:
Summary
Automatically generated
Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.
Read Article
Cite this Article
Gaudreau-Lapierre, A., Garneau, D., Djerir, B., Coulombe, F., Morin, T., Marechal, A. Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation. J. Vis. Exp. (133), e57410, doi:10.3791/57410 (2018).
Copy