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检测梭菌感染后抗原特异性抗体应答的蛋白微阵列测定方法
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09:12 min
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June 15, 2018
DOI:
1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen’s Medical Centre,University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine,Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen’s Medical Centre,University of Nottingham
Summary
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本文介绍了一种简单的蛋白质芯片方法, 用于分析人体血清中高纯度梭菌抗原7丛细胞的体液免疫反应。该协议可推广用于测定多克隆静脉注射免疫球蛋白制剂中特异抗体的反应。
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Cite this Article
Negm, O. H., Hamed, M., Monaghan, T. M. A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection. J. Vis. Exp. (136), e57399, doi:10.3791/57399 (2018).
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