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A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen’s Medical Centre,University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine,Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen’s Medical Centre,University of Nottingham
Chapters
- 00:04Title
- 01:14Preparing a Microarray Plate and Printing with a Contact Robot
- 03:06Array Probing
- 05:19Array Scanning
- 06:36Results: Validation of Protein Microarray to Detect Isotype Specific Antibody Response to Clostridium difficile Toxins and Its Reproducibility
- 08:01Conclusion
This article describes a simple protein microarray method for profiling humoral immune responses to a 7-plex panel of highly purified Clostridium difficile antigens in human sera. The protocol can be extended for the determination of specific antibody responses in preparations of polyclonal intravenous immunoglobulin.
