Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / The MPLEx Protocol for Multi-omic Analyses of…
JoVE Journal
Chemistry

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Chemistry
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
 

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Article DOI: 10.3791/57343-v • 10:12 min • May 30th, 2018
May 30th, 2018 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Biological Sciences Division, Pacific Northwest National Laboratory
* These authors contributed equally

Chapitres

résumé
Automatic Translation

A protocol is presented for simultaneously extracting metabolites, proteins, and lipids from a single soil sample, allowing reduced sample preparation times and enabling multi-omic mass spectrometry analyses of samples with limited quantities.

Tags

MPLEx Protocol Multi-omic Analyses Soil Samples Proteomics Metabolomics Lipidomics Environmental Perturbations Molecular Changes Analytes Sample Preparation Methanol/chloroform-compatible Tubes PTFE Tubes Chloroform-washed Stainless Steel Beads Ultrapure Water Ice Bucket Fume Hood Chloroform To Methanol Ratio Cell Wall Breakdown Enzymatic Activities Tube Vortex Attachments Negative 80 Degree Celsius Freezer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter