Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Journal

Biologie du développement

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Biologie du développement

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Journal JoVE Biologie du développement

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

DOI:

10.3791/57290-v

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09:38 min

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February 20, 2018

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Renee Wei-Yan Chow*^1,2,3,4, Paola Lamperti*^1,2,3,4, Emily Steed^2,3,4, Francesco Boselli^2,3,4, Julien Vermot^2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104,Centre National de la Recherche Scientifique, ³U964,Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

Chapitres

00:04Titre
00:53Preparing Molds and Mounting Agarose and Embedding Embryos
03:31Photoconversion
05:12Unmounting Photoconverted Embryos
06:20Imaging Photoconverted Cells at Later Embryonic Stages
08:05Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
09:01Conclusion

Summary

Traduction automatique

This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

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Chapitres

Summary

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