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Alliant Optogenetics artificiel microARN pour caractériser les effets du gène Knockdown fonction présynaptique dans les Circuits de neurones intacts
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
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Alliant Optogenetics artificiel microARN pour caractériser les effets du gène Knockdown fonction présynaptique dans les Circuits de neurones intacts

9,896 Views

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09:17 min

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March 14, 2018

DOI:

10.3791/57223-v

DOI:
10.3791/57223-v

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09:17 min
March 14, 2018

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3 Views
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1Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

Tags

OptogeneticsArtificial MicroRNARNA InterferencePresynaptic FunctionNeuronal CircuitsSynaptic PhysiologyPresynaptic ProteinsNeuronal CultureGene KnockdownAcute Brain SlicesIn VivoCorticoculturingAraCRNA ExtractionChloroform

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Thalhammer, A., Jaudon, F., Cingolani, L. A. Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits. J. Vis. Exp. (133), e57223, doi:10.3791/57223 (2018).

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