Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Generation of Monoclonal Antibodies Against Natural Products
10,657 Views
•
12:15 min
•
April 06, 2019
DOI:
1School of Life Science,Beijing University of Chinese Medicine, 2Third Affiliated Hospital,Beijing University of Chinese Medicine, 3School of Basic Medical Sciences,Beijing University of Chinese Medicine, 4School of Chinese Materia Medica,Beijing University of Chinese Medicine, 5Center of Scientific Experiment,Beijing University of Chinese Medicine
Summary
Automatically generated
This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.
Read Article
Cite this Article
Zhang, Y., Cao, P., Lu, F., Yan, X., Jiang, B., Cheng, J., Qu, H. Generation of Monoclonal Antibodies Against Natural Products. J. Vis. Exp. (146), e57116, doi:10.3791/57116 (2019).
Copy